In vivo Functional Grade Antibodies

 

Preclinical Functional Grade

The Assay Genie in vivo functional-grade antibodies are high purity, low endotoxin monoclonal antibodies that can be used in translational & preclinical animal studies as well as in vitro functional assays. These antibodies have the highest quality standards in the industry with no detectable leachable protein A or aggregates, extremely low levels of endotoxin and up to ≥ 98% purity.   

The Assay Genie in vivo antibodies are produced in a cGMP and ISO 9001:2015 / ISO 13485:2015 certified facility & can be used for injection into animals on account of being pathogen-free (IDEXX Impact I PCR mouse pathogen profile). 

This range of low- and ultra-low endotoxin antibodies have been tested for use in functional studies such as co-stimulation, depletion, blocking & neutralization experiments as well as applications such as ELISA, FC, ICC, IHC-PE, IHC-FF, IP, WB, ELISpot, CODEX® & CyTOF®. 

The antibodies come in flexible pack sizes including 1mg, 5mg, 25mg, 50mg & 100mg pack sizes with custom packaging available on request too. 

The catalog includes top targets such as immune checkpoint antibodies like PD-1 [clone RMP1-14], PD-L1 (B7-H1) [clone 10F.9G2], and CTLA-4 [9H10], as well as key targets for immune cell depletion e.g CD4, CD8 and many more. 

 

Specifications 

in vivo mAbs 

Low Endotoxin 

in vivo mAbs 

Ultra Low Endotoxin 

Binding validation determined by WB, FC or ELISA YesYes
Endotoxin level determined by the LAL method ≤ 1.0 EU/mg ≤ 0.5 EU/mg
Antibody aggregation screening by analytical SEC ≥ 95% monomer ≥ 98% monomer 
Purity by SDS PAGE 

≥ 95% 

≥ 98% 
Formulation for in vivo use 

No Preservatives 

No stabilizers 

No carrier protein 

Sterile PBS pH 7.2 - No K+ or Ca2+ 

Concentration: > 5mg/mL 

No Preservatives 

No stabilizers 

No carrier protein 

Sterile PBS pH 7.2 - No K+ or Ca2+ 

Concentration: > 5mg/mL 

Murine pathogen ScreeningNot applicable, see Ultra Low Endotoxin functional grade antibodies Pathogen tested (IMPACT1) 
Applications  

In vivo functional studies and may be used ffor WB, FC, IF or IHC

Product preparation 

Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G. to assure extremely low levels of endotoxins, leachable protein A or aggregates. 

Storage and handling 

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 

• 1 month, 2 to 8 °C under sterile conditions, as supplied. 

• 12 months, -70 °C under sterile conditions.

Ultra Low Endotoxin In vivo mAbs


Extremely low endotoxin levels (≤ 0.5 EU/mg - ≤ 1 EU/mg) for in vivo mouse studies or sensitive
in vitro experiments

Validated


Immune checkpoint blockade, neutralization, stimulation, cell depletion, ELISA, FC, ICC, IHC, IP, WB, CODEX®, CyTOF®

Highly Pure


No preservatives, stabilizers, carrier proteins & made using sterilized PBS without K+ and Ca2+.

Super Consistent


cGMP-grade & extensively quality controlled with minimal protein A leaching and low aggregation ensuring excellent Lot-to-Lot reproducibility

 

Product nameCat. no.

Anti-Mouse PD-1 (CD279) [29F.1A12] In Vivo Antibody - Low Endotoxin

AG-IVMB0031

Anti-Human CD3 [UCHT-1] In Vivo Antibody - Low Endotoxin

AG-IVMB0117

Anti-Mouse CD4 [GK1.5] In Vivo Antibody - Low Endotoxin

AG-IVMB0060

Anti-Mouse CD8b.2 In Vivo Antibody - Low Endotoxin

AG-IVMB0093

Anti-Mouse CD8a (Ly 2) In Vivo Antibody - Low Endotoxin

AG-IVMB0068

Mouse IgG1 Isotype Control [HKSP] - Low Endotoxin

AG-IVMB0184

Mouse IgG2a Isotype Control - Low Endotoxin

AG-IVMB0188

Rat IgG2b Isotype Control - Low Endotoxin

AG-IVMB0200

Rat IgG2a Isotype Control - Low Endotoxin

AG-IVMB0199
 

 

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