Seq What You’ve Been Missing— with InTraSeq™ Single Cell Analysis
4. April 2025
Simultaneously Detect RNA, Intracellular, and Surface Proteins for Thousands of Cells at a Single-Cell Level
Intracellular Protein and Transcriptomic Sequencing (InTraSeq) is a novel technology that identifies signaling pathways and reveals molecular mechanisms in disease development in a single experiment. It enables simultaneous detection of RNA as well as both intracellular and surface proteins in thousands of cells, allowing researchers to investigate signaling pathways along with the transcriptome—all at a single-cell resolution.
Developed and validated by CST, InTraSeq 3’ technology uses the 10x Genomics Chromium Single Cell 3’ Reagent Kits with Feature Barcoding technology.
Illustration above:
A) Most single cell analysis techniques currently available detect RNA and use barcoded antibodies to measure surface proteins for phenotyping purposes.
B) Many have tried developing methods to detect intracellular proteins, but these methods typically suffer from significant RNA loss and degradation.
C) InTraSeq™ Single Cell Analysis detects RNA alongside intracellular and surface proteins while preserving RNA.
InTraSeq Benefits
Reduce hands-on time with a streamlined workflow.
A straightforward, four-step protocol with multiple stopping points increases workflow flexibility and shortens hands-on benchwork to approximately one hour.
Investigate intracellular signaling while guaranteeing robust RNA signal
InTraSeq reagents let antibodies enter the cell and bind to targets while maintaining RNA levels for accurate quantitation of both RNA and protein expression.
Identify hard-to-detect cells with unbiased depth of coverage.
Detect and quantify intracellular and surface proteins in conjunction with single-cell gene expression to differentiate heterogeneous cell populations.
Explore multiple molecular mechanisms in one experiment.
Simultaneous measurements of transcriptomics changes and protein expression, including post-translational modifications, generate more comprehensive data sets.
Learn more about IntraSeq in our upcoming webinar & in this poster walkthrough
Webinar: Unlocking The Molecular Mechanism of Pathogenic and Non-pathogenic Th17 cells with InTraSeq.
📅 April 23rd | 15.00 -16.00 CEST
PROGRAMME:
15:00 - 15:05: Introduction
15:05 - 15:25: Dr. Maxime Jacquet introduces CST, validation and InTraSeq
15:25 - 15:50: Presentation by Majd Ariss, PhD, MS
15:50 - 16:00: Q&A
Poster walkthrough
© 2024 Cell Signaling Technology, Inc. CST, Cell Signaling Technology, and InTraSeq are registered trademarks of Cell Signaling Technology, Inc. 10x Genomics, 10x, Feature Barcode, and Chromium are the trademarks or registered trademarks of 10x Genomics, Inc. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information. Subject to patents licensed from 10x Genomics, Inc., for use with single-cell (i.e., Chromium) 10x products.
