SPECIAL PRICE 

Special Price on 3D Organoid Cell Viability Assay and Rapid Mycoplasma Detection Kit

The 3D Organoid Cell Viability Assay is a highly sensitive, 1-step kit to quantify the number of live viable cells directly in 2D cell culture supernatants and 3D Organoid models.

The MycoGenie Rapid Mycoplasma Detection Kit enables the rapid and simple detection of mycoplasma contamination in cell culture.

Use code: GeniePromoQ324

Valid until 31.09.2024

3D Organoid Cell Viability Assay

1-Step, No Wash Assay

  • Quantitative Viability Assay: Quantify viable cells directly in 2D & 3D Organoid models
  • 3D Organoid Validated: Tested with Apical-out Airway organoids & Neuronal organoids
  • 1-Step, No Wash Assay: Homogenous assay with single optimized WST-8 reagent
  • Kinetic Results: Measure Viability, Proliferation or Cytotoxicity from 1 to 48 hours
  • Low Cytotoxic Profile: Very low toxicity versus reagents such as MTS, MTT or PrestoBlue

The Assay Genie 3D Organoid Cell Viability Assay is a highly sensitive, 1-step kit to quantify the number of live viable cells directly in 2D cell culture supernatants and 3D Organoid models.This kit is an excellent choice for proliferation and cytotoxicity studies and been validated in 2D cell culture as well as 3D Apical-Out airway organoids in a high-throughput 384-well format. This technology is based on the reduction of WST-8 Tetrazolium salt by mitochondrial dehydrogenases to an orange product which is directly proportional to the number of viable cells when measured at 450nm. WST-8 is highly soluble in cell culture medium with a low cytotoxicity profile and can be added directly to cultured cells with no pre-treatments or wash steps. It is extremely powerful for high-throughput and sensitive experiments especially those requiring longer incubation steps such as 24 hour or 48 hours. This kit offers a much higher sensitivity and lower cytotoxicity than other Tetrazolium reagents such as MTS, MTT, PrestoBlue or XTT.

Overview

Name

3D Organoid Cell Viability Assay

Art. no. 

AG-AKES081

Size

100 Tests, 500 Tests

Application 

Viability/Proliferation/Cytotoxicity

Detection Method 

Colorimetric Method

Sample Type 

Cell sample

Detection Instrument 

Microplate Reader

3D Apical-out organoids were treated with 0.1% DMSO and cell viability measured with either Assay Genie 3D Organoid Cell Viability Assay or Competitor T reagent after 1h, 3h and overnight incubations. Cell viability obtained with the 3D Organoid Cell Viability Assay on the organoids was above 100% at all time-points versus reduced viability when using PrestoBlue reagent.

 

 

3D Apical-out organoids were treated with 10% DMSO to initiate cell death and viability was measured with either Assay Genie 3D Organoid Cell Viability Assay or Competitor T reagent after 1h, 3h and overnight incubations. Cell viability obtained with the 3D Organoid Cell Viability Assay on the organoids detected reduced viability ranging from 0.5% to 5.9%.

3D Organoid Cell Viability Assay

Description

Pack size

Product no. 

3D Organoid Cell Viability Assay (100 Assays)100 AssaysAG-AKES081-100
3D Organoid Cell Viability Assay (500 Assays)500 AssaysAG-AKES081-500

MycoGenie Mycoplasma Detection Kit

  • Visual Mycoplasma Detection Detect Mycoplasma with a simple visual color change!
  • Rapid Cell Culture Results 1-step, 60-minute assay with no special instrumentation required
  • Highly Sensitive Assay: Detect as little as 500cfu Mycoplasma in 1ul of cell culture supernatant
  • Widely Validated: Detect up to 28 Mycoplasma species that contaminate cell cultures
  • Cost-Effective: Low-cost alternative to PCR, QPCR & ELISA detection methods

The MycoGenie Rapid Mycoplasma Detection Kit enables the rapid and simple detection of mycoplasma contamination in cell culture. This novel system detects mycoplasma in 1 hour from 1µl of cell culture supernatant by visual determination eliminating the requirement for PCR, qPCR, electrophoresis or ELISA. Compared with PCR, the MycoGenie Rapid Mycoplasma Detection Kit is more resistant cell culture inhibitors thus avoiding false positive and false negative results. The results are highly consistent with the most sensitive and accurate PCR kits for Mycoplasma detection.

The MycoGenie Rapid Mycoplasma Detection Kit can detect up to 28 mycoplasma species, including 8 of the most common species associated with cell culture contamination. It is suitable for mycoplasma detection in a wide range of suspension and adherent cells including CHO, Vero, hybridoma, Sf9, HEK293 and many more. Furthermore, the kit is compatible with a comprehensive suite of cell culture media and serum.

The MycoGenie Rapid Mycoplasma Detection Kit is an excellent choice for routine mycoplasma detection in biopharma, vaccine/monoclonal antibody production, cell therapy/embryo laboratories and other scientific research laboratories.

Tube 1. Negative Control

Tube 2. Positive Mycoplasma Control

Tube 3. Positive Mycoplasma Sample

Tube 4. Mycoplasma Elimiated Sample

 

 

MycoGenie Mycoplasma Detection Kit

Description

Pack size

Product no. 

MycoGenie Rapid MycoPlasma Detection Kit20 TestsAG-MORV0011
MycoGenie Rapid MycoPlasma Detection Kit50 TestsAG-MORV0011-50

Want to know more?

Here are the technical manuals if you want to know more.

Isabelle Westerlund, PhD

Product Specialist & Marketing Coordinator

+46 (0)8 588 931 23

isabelle.westerlund@bionordika.se

Isabelle Westerlund, PhD

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