Get the cheapest for free
Buy 3 NEB amplification products - pay for only 2!
Order three reagents from the same group (either Luna, Q5 or OneTaq) and pay only for two. Cannot be combined with other discounts or agreements.
Use code 3f2EU when placing your order to get the discount.
Luna® Universal qPCR & RT-qPCR
Luna® Universal qPCR & RT-qPCR products from New England Biolabs are designed for fluorescence-based quantitative polymerase chain reaction (qPCR) and reverse transcription qPCR (RT-qPCR). These reagents are optimized for high sensitivity and specificity in the detection and quantification of nucleic acids. Luna products are available for both intercalating dye and probe-based detection methods.
Additionally, Luna reagents include the passive reference dye ROX, which is used to normalize fluorescence signals and improve data accuracy. All Luna products are compliant with the MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) guidelines, ensuring high-quality and reproducible qPCR results.
- Luna Universal qPCR Master Mix (#M3003): A ready-to-use 2X master mix for dye-based qPCR, providing high sensitivity and specificity for nucleic acid quantification.
- Luna Universal Probe qPCR Master Mix (#M3004): A 2X master mix optimized for probe-based qPCR, ensuring robust and reliable performance with high sensitivity.
- Luna Universal One-Step RT-qPCR Kit (#E3005): An all-in-one kit for one-step RT-qPCR, combining reverse transcription and qPCR in a single tube for efficient and sensitive detection.
- Luna Universal Probe One-Step RT-qPCR Kit (#E3006): A comprehensive kit for probe-based one-step RT-qPCR, designed for high efficiency and sensitivity in a single-tube format.
- Luna Probe One-Step RT-qPCR 4X Mix with UDG (#M3019): A 4X master mix for probe-based one-step RT-qPCR, including UDG for carryover prevention, providing high sensitivity and specificity.
- LyoPrime Luna® Probe One-Step RT-qPCR Mix with UDG (#L4001): A lyophilized mix for probe-based one-step RT-qPCR, incorporating UDG for carryover prevention, offering convenience and stability for high-performance assays.
The Luna reagents have been cited in 6200+ publications – order your reagents today with our special 3 for 2 offer!
OneTaq® DNA Polymerase
The OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases, designed for robust amplification across a wide range of templates. The 3′–5′ exonuclease activity of the Deep Vent DNA Polymerase enhances the fidelity and robustness of Taq. The OneTaq Reaction Buffers and High GC Enhancer are formulated to provide robust yields with minimal optimization, regardless of the template's GC content. The OneTaq polymerase is available in a master mix and a Quick-Load® master mix format.
- OneTaq Hot Start DNA Polymerase (#M0481)
Utilizes aptamer technology for hot start, which binds to the polymerase through non-covalent interactions, blocking its activity at temperatures below 45°C. This means that the polymerase is activated during normal cycling conditions, allowing reactions to be set up at room temperature. This feature combines convenience with decreased interference from primer dimers and secondary products.
- OneTaq Quick-Load 2X Master Mix with Standard Buffer (#M0486)
Contains an optimized blend of Taq and Deep Vent® DNA Polymerases in a convenient master mix format, as well as two commonly used tracking dyes for DNA gels.Suitable for direct and fast agarose gel loading after routine PCR, such as genotyping and colony PCR.
- OneTaq Quick-Load DNA Polymerase (#M0509)
Features: Supplied with a green 5X OneTaq Quick Load Reaction Buffer in addition to the colorless 5X OneTaq Reaction Buffer. 2x higher fidelity than Taq and designed for direct and fast agarose gel loading after routine PCR.
Q5® High-Fidelity DNA Polymerase
The Q5® High-Fidelity DNA Polymerase delivers unmatched accuracy with significantly higher fidelity compared to Phusion, making it perfect for demanding applications. It ensures high specificity and yield across a wide range of templates, including those with high GC and AT content. Additionally, the Q5 Blood Direct 2X Master Mix enables direct PCR from blood samples, streamlining workflows by eliminating the need for DNA purification.
- Q5® High-Fidelity DNA Polymerase (#M0491)
Q5® High-Fidelity DNA Polymerase sets a new standard for fidelity and performance, offering over 280 times higher fidelity than Taq polymerase. It is fused with the Sso7d DNA binding domain, enhancing speed, fidelity, and reliability
- Q5® Hot Start High-Fidelity DNA Polymerase (#M0493)
This variant utilizes engineered oligonucleotides known as aptamers to block enzyme activity during setup, which is dissociated during normal cycling conditions. This allows for room temperature setup without a high-temperature activation step, reducing reaction times
- Q5 Master Mixes (#M0492, #M0494)
These master mixes contain dNTPs, Mg++, and a proprietary buffer, requiring only the addition of primers and DNA template. They are designed for robust amplification regardless of GC content
- Q5U® Hot Start High-Fidelity DNA Polymerase (#M0515)
This modified version of Q5 High-Fidelity DNA Polymerase includes a mutation in the uracil-binding pocket, enabling it to read and amplify templates containing uracil and inosine bases. It is useful for amplifying bisulfite-converted, enzymatically-deaminated, or damaged DNA, and for preventing carryover contamination in PCR
- Q5 Blood Direct 2X Master Mix (#M0500)
This product can amplify a wide variety of targets directly from dried blood spots or up to 30% whole human blood, eliminating the need for DNA purification
Benedikt von der Heyde, PhD
Product Specialist
NGS & SciLifeLab
